Coding

Part:BBa_K3807036:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


gRNA scaffold with riboswitch protospacer 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

enCas9 protospacer designed to bind at the 5' end of the TetA gene (directly downstream of the riboswitch sequence) merged to enCas9 gRNA body that we took from the original pEvolvR plasmid.

Source

Modified from a part amplified from Addgene plasmid #113077

References

Halperin et al, 2018